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90.225 cents
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The Pythagorean limma, or Pythagorean semitone, is the interval of size 256/243 = 2^8/3^5 (about 90.2¢), which is the diatonic semitone in Pythagorean tuning. It can be generated by stacking five 4/3 just perfect fourths and octave-reducing the resulting interval.

See: Gallery of Just Intervals, comma

5edo, 10edo, 15edo, 20edo, 25edo and 30edo temper it out.